Fig. 1

Discovery cohort gene expression clusters (A), and association with TIGS clusters (B), CD8 IHC patterns of T-cell infiltration (C–E), and TIGSdistribution within CD8 cohort (F). A. Unsupervised clustering of 1323 clinical RNA-seq profiles yield three immunogenic clusters, namely, inflamed (n = 439/1323; 33.18%), borderline (n = 467/1323; 35.30%) and non-inflamed (n = 417/1323; 31.52%). The tumor immunogenic signature (TIGS) cluster of genes contains 161-genes that are over-represented by T & B cell activation pathways along with IFNg, chemokine, cytokine and interleukin pathways. Mean expression of the 161 genes constituting the TIGS cluster produces the TIGS score. B. Distributions of the TIGS of the samples in each of the three sample clusters. C-E. Representative CD8 immunohistochemistry images of T cell infiltration patterns of Infiltrating (C), Non-infiltrating (D), and excluded (E). F. The distribution of immunogenic scores for tumors in the discovery cohort with strongly infiltrating, non-infiltrating, and excluded CD8 T cell infiltration patterns