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Fig. 3 | Biomarker Research

Fig. 3

From: Expression of immune checkpoints on circulating tumor cells in men with metastatic prostate cancer

Fig. 3

Representative patient CTCs stained for cytokeratin (CK), 4′,6-diamidino phenylindole (DAPI), CD45 (marker of leukocytes), and the immune checkpoint ligand of interest ([A] PD-L1, [B] PD-L2, [C] B7-H3, or [D] CLTA-4). CTCs are defined as being CK-positive, DAPI-positive, and CD45-negative. Orange boxes are indicators from the CellTracks Analyzer II that a cell was identified as marker-positive

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