Fig. 1

Dissection and clustering of AML patient BM cells and healthy donor BM cells. a The UMAP projection of BM cells from 16 AML patients and 4 healthy donors, showing the formation of 22 main clusters, including 3 for T/NK cells (Cluster 0, Cluster 3, and Cluster 17), 3 for B lineage (Cluster 12, Cluster 14, and Cluster 16), 3 for mature myeloid lineage (Cluster 1, Cluster 11, and Cluster 20), 3 for erythroid lineage (Cluster 8, Cluster 18, and Cluster 19), 9 for SP-like cells (Cluster 2, Cluster 4, Cluster 5, Cluster 6, Cluster 7, Cluster 9, Cluster 10, Cluster 13, and Cluster 15), and 1 for non-hematopoietic stromal cells (Cluster 21). Each dot represents to one single cell, and is colored according to cell cluster. b CD3E, CD4, CD8A, CD1C, CD14, NCAM1, and CD79B-expressing (expression value > 0) of across 36,477 BM-derived cells illustrated in UMAP plots. c Histogram of cell-type fractions for each AML patient and healthy donors’ BM cells, colored based on cell type