Fig. 2
From: Identification of pAKT as a pharmacodynamic marker for MER kinase in human melanoma G361 cells
MER kinase is required for GAS6–induced pAKT in G361 cells. a Western blot showing knockdown of MER and TYRO3 in G361 cells. b Knockdown of MER fully inhibits growth arrest–specific protein 6 (GAS6)-induced phosphorylated AKT (pAKT) in G361 cells. Results are shown as mean ± standard deviation (SD) of 3 wells (*p < 0.05). c TYRO3 knockdown did not affect total MER and pAKT in G361 cells upon GAS6 treatment. Results are shown as mean ± SD of 3 wells (*p < 0.05). A mixture of 4 small, interfering RNAs was used per gene to knockdown AXL, MER, and TYRO3. DMSO: dimethyl sulfoxide; NTC: non-targeting control