Fig. 2

Fluvastatin and atorvastatin inhibit YT-INDY proliferation and cytotoxicity. Control cells were treated with the solvents in which the drugs were dissolved. All compounds were added to the cells at the start of the experiment. Each experiment was performed at least four times and the data was analyzed for statistical significance by Student’s t-test. Statistical significance at p < 0.05 is denoted by an asterisk. a YT-INDY cells were incubated for 72 h in the presence of fluvastatin or atorvastatin, in the presence or absence of mevalonate. These results show that fluvastatin and atorvastatin can inhibit YT-INDY cell growth and that mevalonate can reverse the effects of the statins, demonstrating that the major effect is taking place within the mevalonate pathway. b YT-INDY cells were incubated for 24 h with fluvastatin or atorvastatin, in the presence or absence of mevalonate or geranylgeranyl pyrophosphate, and tested in a cellular cytotoxicity assay. Control levels of YT-INDY cytotoxicity against the target cell line averaged 61.6% ± 3.32 (standard error of the mean) (n = 16 experiments). Experimental results were compared to control results to derive the percent inhibition of cytotoxicity. Fluvastatin and atorvastatin potently inhibited the cellular cytotoxicity of YT-INDY cells, whereas mevalonate and geranylgeranyl pyrophosphate could nearly completely restore cytotoxic activity