Fig. 4
From: Kinomic profiling of glioblastoma cells reveals PLCG1 as a target in restricted glucose
Kinetic curves for peptides corresponding to regions of PLCG1 and RAF1. Kinomic profiling demonstrated changes in the peptides that contain tyrosine phosphorylation sites corresponding to regions of PLCG1 (a, b) and Raf-1 (c, d). Representative curves for results from lysates isolated from GBM14 (a, c) or GBM456 (b, d) cells cultured in standard or restricted glucose with measurements over pumping cycles (time) are shown. Phosphorylation as measured by FITC intensity was captured at the indicated cycle number as the lysate was pumped across the array in cycles