Table 2 Expression of 21 selected miRNAs detected by three different methods
From: Elevation of brain-enriched miRNAs in cerebrospinal fluid of patients with acute ischemic stroke
miRNA | NGS | Exiqon qPCR | Applied Biosystems qPCR | ||||||
|---|---|---|---|---|---|---|---|---|---|
Detection(%) | Stroke/Control FC (P) | Large/Small FC (P) | Detection(%) | Stroke/Control FC (P) | Large/Small FC (P) | Detection(%) | Stroke/Control FC (P) | Large/Small FC (P) | |
let-7b-5p | 95 | 0.8 (0.99) | 2.3 (0.73) | 98 | 1.1 (0.99) | 0.6 (0.29) | 100 | 1.0 (0.89) | 0.6 (0.57) |
let-7c-5p | 100 | 0.7 (0.99) | 0.9 (0.99) | 74 | 0.7 (0.99) | 0.6 (0.11) | 95 | 0.7 (0.71) | 0.3 (0.88) |
miR-9-5p | 98 | 2.8 (0.06) | 7.0 (0.00) | 48 | 3.3 (0.90) | 2.3 (0.07) | 100 | 0.9 (0.95) | 10.0 (0.01) |
miR-9-3p | 70 | 7.1 (0.08) | 6.8 (0.28) | 70 | 1.4 (0.85) | 9.8 (0.01) | 100 | 1.2 (0.73) | 1.9 (0.66) |
miR-15a-5p | 63 | 1.0 (0.99) | 2.0 (0.99) | 100 | 1.0 (0.50) | 2.2 (0.31) | 100 | 1.0 (0.96) | 2.4 (0.44) |
miR-30d-5p | 100 | 1.2 (0.99) | 1.3 (0.79) | 91 | 1.6 (0.45) | 1.1 (0.48) | 100 | 1.7 (0.01) | 6.2 (0.09) |
miR-99b-5p | 100 | 1.9 (0.32) | 4.2 (0.00) | 38 | 0.7 (0.99) | 0.9 (0.84) | 72 | 0.4 (0.43) | 0.5 (0.70) |
miR-103a-3p | 100 | 1.9 (0.17) | 4.3 (0.00) | 48 | 1.0 (0.84) | 0.4 (0.80) | 100 | 0.6 (0.49) | 0.9 (0.85) |
miR-107 | 100 | 2.0 (0.13) | 4.5 (0.00) | 31 | 0.6 (0.71) | n.d. small | 100 | 3.6 (0.37) | 3.5 (0.58) |
miR-124-3p | 58 | 2.7 (0.99) | 11.0 (0.19) | 83 | 0.9 (0.99) | 3.6 (0.03) | 100 | 1.1 (0.92) | 1.7 (0.74) |
miR-128-3p | 100 | 2.5 (0.04) | 5.5 (0.00) | 41 | 1.0 (0.95) | 2.7 (0.15) | 100 | 0.6 (0.42) | 1.5 (0.88) |
miR-129-5p | 60 | 2.6 (0.99) | 2.3 (0.99) | 26 | 1.1 (0.91) | 3.1 (0.14) | 100 | 1.5 (0.97) | 1.0 (0.77) |
miR-181a-5p | 100 | 1.5 (0.40) | 3.0 (0.00) | 33 | 0.9 (0.78) | 0.6 (0.49) | 100 | 0.5 (0.33) | 0.7 (0.76) |
miR-181b-5p | 100 | 1.8 (0.32) | 3.1 (0.02) | 5 | n.d. control | n.d. small | 100 | 0.9 (0.46) | 0.7 (0.58) |
miR-203a-3p | 100 | 0.3 (0.02) | 0.8 (0.99) | - | - | - | 100 | 0.7 (0.85) | 1.4 (0.78) |
miR-204-5p | 100 | 0.8 (0.99) | 0.6 (0.73) | 100 | 0.8 (0.90) | 0.6 (0.13) | 100 | 0.8 (0.46) | 0.7 (0.06) |
miR-210-3p | 38 | 1.3 (0.99) | 2.3 (0.99) | 38 | 1.1 (0.99) | 0.4 (0.06) | 100 | 0.9 (0.52) | 1.0 (0.81) |
miR-221-3p | 95 | 1.0 (0.99) | 3.0 (0.63) | 79 | 1.1 (0.97) | 0.3 (0.33) | 81 | 0.7 (0.32) | 0.4 (0.52) |
miR-485-5p | 72 | 2.4 (0.99) | 5.5 (0.46) | - | - | - | 100 | 0.9 (0.54) | 2.2 (0.73) |
miR-497-5p | 28 | 2.9 (0.99) | 2.6 (0.99) | 74 | 1.0 (0.94) | 4.1 (0.32) | 79 | 1.6 (0.44) | 3.9 (0.65) |
miR-1246 | 78 | 11.5 (0.02) | 13.6 (0.01) | - | - | - | 100 | 1.2 (0.40) | 1.0 (0.80) |