Fig. 2

Mantle cell lymphoma uses a different polyadenylation sequence than other cancer cells. a PCR products derived from 3′RACE of mRNA isolated from MCF7 and Jeko-1. C1 represents minus reverse transcriptase controls and C2 are controls minus the oligo(dT25)T7 primers. The blue line was inserted to highlight the slight difference in the size of the two 3′UTR transcripts. The method for 3′RACE was previously described [5]. b A partial sequence of the 3′UTR cDNA sequence derived from MCF7 compared to that of Jeko-1 showing the polyA tail (in green) for each transcript together with the PAS (underlined). Highlighted in yellow are differences in the nucleotide sequences between the two sequences, upstream of the first cleavage and polyadenylation site