Fig. 1

miRNA-seq analysis results. Samples were drawn at four timepoints: (T1) before conditioning chemotherapy, (T2) on the day of AHSCT (day 0), day + 7 (T3), and + 14 day after AHSCT (T4). (A) heatmap of miRNAs differently expressed across study timepoint assessed by repeated measures ANOVA. The serum miRNA profiles tend to cluster by the study time points- two clusters- “early” (T1 and T2) and “late” (T3 and T4) are visible. One minus Pearson correlation distance metric and complete linkage method were used. (B-H) Plots for seven miRNAs differentially expressed across AHSCT procedure in miRNA-seq stage of the study. There were no statistically significant results in the comparison of miRNAs expression between at T1 and T2. In T3, hsa-miR-320c (B) was significantly upregulated compared to T1 (FC = 3.92, p = 0.007), whereas hsa-miR-223-3p (C) was significantly downregulated (FC 0.31, p = 0.048). MiRNA levels at T4, in comparison to T1, showed significant downregulation of both hsa-let-7f-5p (D) and hsa-miR-155-5p (E) (FC = 0.38, p = 0.004 and FC = 0.37, p = 0.019, respectively), while hsa-miR-320c (B) was significantly upregulated (FC = 2.39, p = 0.049). At T3, there was lower expression of hsa-miR-18a-5p (F) (FC = 0.16, p = 0.035) and hsa-miR-223-3p (C) (FC = 0.35, p = 0.033) comparing to T2. Comparing T4 with T3, a lower expression level of hsa-miR-486-5p (G) (FC = 0.72, p = 0.024) was identified. In a comparison of T4 with T2, a higher expression level of hsa-miR-96-5p (H) (FC = 8.33, p = 0.036) was observed. Asterisks denote the significance level (paired t-test with Bonferroni correction): *- p ≤ 0.05; **- p ≤ 0.01. (I-J) Volcano plots showing differentially expressed miRNAs in patients with platelet delayed engraftment (DE) (I) and neutrophil DE (J). Red dots represent upregulated miRNAs; blue dots represent downregulated miRNAs; grey dots represent miRNAs with no significant difference. (K) Volcano plot showing differentially expressed miRNAs in patients who developed bacteremia. Only miRNAs in T1 and T2 (before the event occurrence) were included in the analysis to establish potential predictors for further classifier development