Fig. 5

The DAB2 scaffolding function regulates YAP1 phosphorylation by promoting ITGB3-SRC interaction. A-B Western blot for active-RHOA, p-SRC (Y416), RHOA, and SRC were performed in GC cell lines treated with H pylori strains 26,695 and 43,504. C-D Western blot for active-RHOA, p-SRC (Y416), RHOA, and SRC were performed in gastric cancer cell lines with DAB2 overexpression or knockdown. E Western blot analysis for active-RHOA, p-SRC (Y416), RHOA, SRC, p-YAP1 (Y357), and YAP1 were performed in GC cell lines after treatment with SRC inhibitor dasatinib. F Physical interactions among DAB2, ITGB3, and SRC were predicted by STRING database (http://string-db.org/). G Co-immunoprecipitation of SRC and ITGB3 in AGS cells with or without DAB2 knockdown (left) and in HGC27 cells with or without DAB2 overexpression (right). H Representative IF images (magnification ×400, scale bars, 50 μm) of DAB2, ITGB3, and SRC in uninfected and H pylori 26,695–infected organoids derived from normal human gastric tissues; nuclei were stained with DAPI (blue). I Representative IF images (magnification ×400, scale bars, 20 μm) of P-STAT3, active-YAP1, and Ki67 in uninfected and H pylori 26,695–infected PDOs; nuclei were stained with DAPI (blue). J-K The quantification of nuclear Ki67 (J) and active-YAP1 (K) fluorescence is shown as the mean ± standard deviations of 3 independent fields; **P < 0.01