Fig. 4

H pylori activated YAP1 signaling via DAB2. A Spearman’s correlation between DAB2 and YAP1 signaling genes (AXL, CTGF and CYR61) was examined in the TCGA cohort. B The expressions of YAP1 signaling genes (AXL, CTGF and CYR61) were examined in AGS cells with DAB2 overexpression or knockdown (*P < 0.05; **P < 0.01). C IF analysis shows the co-expression of DAB2 and active-YAP1 in patients with atrophic gastritis, intestinal metaplasia, and dysplasia (magnification ×200, scale bar, 50 μm). Quantification scores are shown on the right panel (*P < 0.05; **P < 0.01). D-E Western blot for DAB2, active-YAP1, p-YAP1 (S357), p-YAP1 (S127), and total YAP1 were performed in GC cells with DAB2 overexpression or knockdown. F Western blot for DAB2, active-YAP1, p-YAP1 (S357), p-YAP1 (S127), and total YAP1 in AGS and HGC27 cell following DAB2 silencing and H pylori infection. G Kaplan-Meier survival curve demonstrates an association between high expressions of DAB2 and YAP1 signaling genes (AXL, CTGF and CYR61) and overall survival (OS) in overall GC patients and intestinal-type GC