Fig. 3

DAB2 expression is induced via direct transcriptional activation of STAT3 on the DAB2 promoter. A-B DAB2 promoter luciferase reporter assays were performed in AGS and HGC27 cells with IL6 treatment (A) or H pylori infection (26,695 and 43,504, B); **P < 0.01, ***P < 0.001. C-D A schematic diagram shows the location of STAT3 putative binding regions on DAB2 promoter. ChIP assay primers were designed to cover regions P1 to P3. E ChIP assay showing amplification of DNA fragments quantified by qRT-PCR and demonstrating the binding of STAT3 on the DAB2 promotor in P1 region. Data are expressed as the means ± standard deviations. **P < 0.01. F AGS cells with IL6 treatment and infection of H pylori strains (26,695 and 43,504). ChIP assay using STAT3 antibody was performed, followed by qPCR applying primers covering P1 region; ***P < 0.001. G Spearman’s correlation between DAB2 and IL6 was examined in the TCGA cohort