Fig. 6

Functional characterization of integrated miRNAs in normal lung cell line. Merged mRNA-sequencing datasets were generated from a normal lung cell line (BEAS2B) transfected with individual miR3124_mimic, miR200b_mimic, and miR92b_inhibitor, or mixed sets of 3-miRNAs. (A) Principal component analysis (PCA) plot displaying the merged mRNA-sequencing data sets. (B) Volcano plots illustrating the distribution of significantly up- and down-regulated mRNAs (p < 0.05) by more than 2-fold. (C) Upset plots showing the number of all differentially expressed mRNAs between groups. (D) Heat map showing differentially expressed genes in ERBB, EGF, JAK-STAT, and Apoptosis pathways. The gene ontology (GO) term associated with each gene was indicated with a dot, and (E) the GO term for each pathway is described (ERBB [Orange: A,I,J,M], EGF [Green: B,H,L], JAK-STAT [Sky: C-G,K], and Apoptosis [Red: A-N]). (F) Bubble plot to visualize disease ontology (DO) terms related to lung disease. All bubbles represent gene sets. The larger the bubble, the greater the number of genes in the gene set. (G) DO plot based gene set enrichment analysis (GSEA) of lung cancer terms