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Fig. 5 | Biomarker Research

Fig. 5

From: EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway

Fig. 5

Circ_0001187 acts as a sponge to enhance RNF113A expression via Circ_0001187/ miR-499a-5p/ RNF113A axis. A Heatmap for differentially expressed miRNAs via RNA pulldown assay by probe-Circ_0001187. B RNA FISH showing colocalization of Circ_0001187 and miR-499a-5p in THP-1 and Molm-13 cells. Scale bars, 20um. C RNF113A contains conserved target sites of miR-499a-5p by using the Target Scan and its mutations. D The results of dual-luciferase reporter assay for miR-499a-5p and RNF113A. E The expression levels of RNF113A in THP-1 and Molm-13 cells transfected with miR-499a-5p mimics or mimics/inhibitor respectively through qRT-PCR. F The expression levels of RNF113A in THP-1 and Molm-13 cells transfected with si-Circ or si-Circ/miR-499a-5p inhibitor through qRT-PCR. G Western blot results of RNF113A and METTL3 in THP-1 and Molm-13 cells transfected with si-Circ or si-Circ/miR-499a-5p inhibitor. H The effect of si-Circ or si-Circ/miR-499a-5p inhibitor on the proliferation of THP-1 and Molm-13 cells. I, K The effect of si-Circ or si-Circ/miR-499a-5p inhibitor on the apoptosis of THP-1 and Molm-13 cells. J, L The effect of si-Circ or si-Circ/miR-499a-5p inhibitor on the apoptosis of THP-1 and Molm-13 cells cultured with Ara-C (1 μM, 24 h). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: Not significant

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Biomarker Research

ISSN: 2050-7771

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