Fig. 3

Transient knockdown of YY1 by siRNA in B-NHL. Cell lines RAMOS (a, d), U2932-R2 (b, e) and SC-1 (c, f) were transfected by nucleofection with two different siRNAs targeting YY1 (siYY1_3, siYY1_5) and the combination of both (siYY1_3 + 5), as well as a scrambled siRNA (siSCR) control. a YY1 mRNA quantification relative to GAPDH by qRT-PCR of the 24 h and 48 h post nucleofection samples in RAMOS, b in U2932-R2 and c in SC-1. The scrambled control was set to 1 at each time point. Error bars indicate 95% confidence interval of the mean expression. Asterisks indicate a p-value < 0.05 in case of a significant difference between the mean of a siRNA treated sample compared to the mean of the scrambled control according to t-test. d Western blot analysis of YY1 protein of the same samples shown in (a). e Western blot of YY1 protein of the same samples shown in (b). f Western blot of YY1 protein of the same samples shown in (c). GAPDH was used as loading control. Numbers refer to the relative amount of YY1 normalized to GAPDH levels according to densitometric analyses of the blots. The scrambled control was set to 1 at each time point