Figure 6

Measurements of IL-1 α and IL-1RA from three different skin regions on healthy volunteers on five consecutive days. TAP’s containing capture antibody micro-arrays coated with anti-IL-1α and -IL-1RA capture antibodies were incubated on skin of the inner side of the lower arm (‘Forearm’), cheek (‘Face’) or collar bone (‘Neck’) regions of ten healthy volunteers for 20 minutes. The capturing procedure was repeated on the four following days on exact the same regions, at around the same time-point of day. IL-1α and IL-1RA captured from skin were analyzed in spot-ELISA and signals were quantified by determining the pixel intensities of digitized spots. Signal intensities were compared to signals obtained using fixed concentrations of recombinant IL-1α and IL-1RA, captured in solution. In graph A, the apparent average concentrations of IL-1α black bars and IL-1RA (white bars) have been plotted for the three different body regions. In graph B, the apparent average concentrations of IL-1α black bars and IL-1RA (white bars) have been plotted for the three different body regions for each of the ten persons (1–10 in graphs) analysed. Y-axis: Apparent concentration of IL-1α and IL-1RA on skin in ng/ml. X-axis: Individual participants labeled 1–10. Error bars in graph A represent the standard deviations from average of combined measurements in the 10 healthy volunteers. Error bars in graph B, represent the standard deviations from the average of measurements from five different days for each of the healthy volunteers.