Figure 3

Statins abrogate YT-INDY cytotoxicity against a tumor target cell line in a cytotoxicity assay. A single addition of statin (25 µM, 12.5 uM, 6.2 µM or 3.1 µM), or solvent in which the statins were dissolved, was added to YT-INDY cells at the start of the experiment. After a 24 hour incubation period, the cells were harvested, washed and utilized in the 4 hour cytotoxicity assay. Some statin-treated cells (3.1 µM for each statin) also included the addition of mevalonate (1 mM or 0.5 mM). Statistical analysis was performed using Student's t-test. Panel A demonstrates the inhibition of YT-INDY cytotoxicity compared to the control. All values shown are statistically significant. Panel B shows the reversal of statin-mediated inhibition of cytotoxicity in the presence of mevalonate. All mevalonate values were highly statistically significant.