Outgrowth of a CSF3R-mutant clone drives a second myeloproliferative neoplasm in a chronic myeloid leukemia patient: a case report

Background Chronic myeloid leukemia (CML) and chronic neutrophilic leukemia (CNL) are two myeloproliferative neoplasms with mutually exclusive diagnostic criteria. A hallmark of CML is the Philadelphia chromosome (Ph), which results in a BCR-ABL1 fusion gene and constitutive tyrosine kinase activity. CNL is a Ph-negative neoplasm and is defined in part by the presence of CSF3R mutations, which drive constative JAK/STAT signaling. Case presentation Here, we report the exceedingly rare co-occurrence of two granulocytic myeloproliferative neoplasms in a 69-year old male patient. After an initial diagnosis of chronic myeloid leukemia, the patient’s clinical course was shaped by hematologic toxicity, the emergence of treatment-resistant BCR-ABL1 clones, and the expansion of a CSF3R-mutant clone without ABL1 mutations under selective pressure from tyrosine kinase inhibitors. The emergence of the CSF3R-mutant, neutrophilic clone led to the diagnosis of CNL as a second myeloproliferative neoplasm in the same patient. Conclusions This is the first reported case of CNL arising subsequent to CML, which occurred under selective pressure from targeted therapy in a patient with complex clonal architecture. Patients with such molecularly complex disease may ultimately benefit from combination therapy that targets multiple oncogenic pathways.


Background
Myeloproliferative neoplasms (MPNs) are clonal hematologic malignancies in which a hematopoietic stem cell defect drives proliferation of mature myeloid cell lineages. Chronic myeloid leukemia (CML) and chronic neutrophilic leukemia (CNL) are two MPNs with mutually exclusive diagnostic criteria. A hallmark of CML is the presence of the Philadelphia (Ph) chromosome, which results from t (9;22) and separates CML from Ph-negative MPNs: essential thrombocythemia, primary myelofibrosis, polycythemia vera, chronic neutrophilic leukemia [1]. The BCR-ABL1 fusion gene results from t (9;22) and drives the proliferation of mature myeloid cells through constitutive tyrosine kinase activity [2,3]. In this report, we describe a second rare MPN, chronic neutrophilic leukemia (CNL), arising after a CML patient achieved a complete molecular remission. This is the first reported case of CNL arising subsequent to CML.

Case presentation
A 69-year-old presented initially to his primary care provider with a progressive cough accompanied by weakness, fatigue, and abdominal distension. His white cell count was 113,900/mm 3 , hemoglobin was 11.8 g/dL, and platelet count was 325,000/mm 3 . A bone marrow biopsy showed a markedly hypercellular marrow (100%) with4 % blasts, consistent with chronic phase CML. A 300 cell differential showed left-shifted myelopoiesis in which mid-range differentiated granulocytes-myelocytes, metamyelocytes with lesser amounts of promyelocytes-predominated over more differentiated forms. Myeloid and erythroid precursors were present in a 20:1 ratio. Auer rods were not observed. A Fluorescent In Situ Hybridization (FISH) assay confirmed a BCR-ABL1 translocation and cytogenetics revealed the Ph chromosome resulting from a (9;22) translocation. The major BCR-ABL1 transcript (p210) was 66.45% on the international scale (IS). The patient was started on 300 mg of nilotinib twice daily, which reduced his BCR-ABL1 to 0.403% (IS) in 18 weeks. However, the emergence of six new pathogenic mutations as well as hematologic toxicity of ABL1 inhibitors in this case made the treatment course complex (Tables 1, 2).
After 18 weeks on nilotinib, the patient presented with chest pain and was found to have pericarditis, atrial fibrillation, and severe thrombocytopenia (platelets at 11,000). The patient was ultimately started on imatinib at 400 mg/ day but thrombocytopenia remained problematic. After 2 months, imatinib was reduced to 300 mg/day in an attempt to stabilize the patient's ongoing thrombocytopenia. His platelets only slightly improved, however BCR-ABL1 transcripts decreased. The 400 mg/day dose was resumed when BCR-ABL1 rose from 0.68 to 2.74% (IS); however, subsequent ABL sequencing detected an imatinib and nilotinib-resistant E255V mutation [4], and the patient switched to dasatinib at 100 mg/day.
Despite the disappearance of the Ph chromosome, the patient's white blood cell and absolute neutrophil counts rose while on ponatinib, raising concerns about whether a second Ph-negative MPN could be emerging (Fig. 1). A 220 gene next generation sequencing panel was ordered and two CSF3R mutations, T618I and W818*, were identified at a variant allele frequency (VAF) of 41.1 and 40.3%, respectively. Predicted pathogenic variants were identified based on the COSMIC v92 (Catalogue of Somatic Mutations in Cancer) database. Retrospective analysis of an archived marrow sample revealed that the CSF3R mutations had been present for at least the past 3 years. Bone marrow from diagnosis was not available for retrospective next generation sequencing analyses. In total, six predicted-pathogenic variants were detected in the patient over the course of his treatment, including two CSF3R mutations (T618I and W818*), two ABL1 mutations (E255V and T315I), KMT2C/MLL3 (S1860C), and TET2 (R1167K) ( Table 2).

Discussion and conclusions
This patient had difficulty tolerating tyrosine kinase inhibitors (TKIs), primarily due to hematologic toxicity. While thrombocytopenia is a known side effect of TKIs, the severity and protracted nature of the side effect in this instance suggests dysfunction in the residual BCR-ABL1 negative stem cells. In this case, the emergence of six predicted-pathogenetic variants over the five-year course of treatment suggests that the patient either had a markedly elevated mutational load prior to diagnosiswith different clones emerging in response to TKI selection-or a propensity to develop mutations beyond what is normally associated with aging. The mutations in BCR-ABL1 and TET2 were present at similar variant allele frequencies at 69 weeks and lost during treatment with dasatinib and ponatinib, suggesting they were present in the same clone. Meanwhile, the CSF3R-mutant clone expanded during BCR-ABL1-directed therapy and is likely independent. Given that the increase in neutrophils began when the patient was switched from dasatinib to ponatinib, it is plausible that the expansion of this clone occurred during treatment with ponatinib specifically. It is known that CSF3R truncating mutations are sensitive to dasatinib [14], and that may have contributed to a delayed onset of this second MPN.
It is well established that selective pressure from TKIs allows for the outgrowth of BCR-ABL1 clones harboring point mutations in ABL1. However, this study suggests that the clearance of a BCR-ABL1 positive clone allowed for the outgrowth of a second, genetically distinct leukemia. It is interesting to note that two granulocytic leukemias arose in this patient, suggesting that either cell intrinsic or microenvironmental factors caused a specific predilection for malignancies of the granulocytic lineage. Germline mutations have not been accessed for this patient. This case describes the exceedingly rare cooccurrence of CML and CNL in the same individual and is an unusual example of clonal evolution under selective pressure from targeted therapy. These findings highlight that myeloid disease with complex clonal architecture is prone to molecular evolution. Patients with such molecularly complex disease may ultimately benefit from combination therapy that targets multiple oncogenic pathways. Authors' contributions DB and BJD treated the patient; SAC, TPB and JEM analyzed and/or interpreted the data; SAC and TPB wrote the paper. All authors reviewed and approved the manuscript. Availability of data and materials Data sharing requests should be sent to the corresponding author.

Ethics approval and consent to participate
The OHSU IRB determined that the proposed activity is not research involving human subjects. Access and use of protected health information complies with OHSU HIPAA policies for non-research activities.

Consent for publication
Not applicable. Data presented in this study has been de-identified and contains none of the 18 HIPPA individual identifiers.