Fig. 2
From: Adoptive neoantigen-reactive T cell therapy: improvement strategies and current clinical researches
Feasible improvement for the manufacture of NRT cells. A Optimize neoantigen predicting platforms to promote the efficiency and accuracy of prediction. B Micro-electroporation (a), microinjection (b) and nano-delivery c can be applied to elevate transfection efficiency. Artificial APCs can be used to increase the efficiency of antigen presentation. C Promote NRT cell expansion in rapid expanding protocol (REP) through adding cytokines (IL-2, IL-7 and IL-15) (a) or anti-4-1BB antibody (b), using feeder cells (Bcl-xL, K562) (c), inhibiting AICD signaling cascade (d), or adopting the culture method of “young” T cells (e) or organoids (f). D Screen NRT cells with surface or genetic markers via single-cell transcriptome and TCR sequencing