Fig. 1

Process of NRT cell manufacturing and adoptive therapy. NRT cells are manufactured via the following steps: A acquisition and cultivation of tumor specimens and peripheral blood mononuclear cells; B mutation identification with WES/WGS/RNA sequencing (seq), potential antigen detection with mass spectrometry; C neoantigen prediction; D design and synthesis of neoantigen-encoding mRNA in tandem minigene configuration or neoantigen peptides; E pulsing DCs directly with peptides, or transfection of neoantigen-encoding mRNA into DCs by electroporation, followed by the co-incubation of neoantigen-loaded DCs and PBMC-derived T cells, F flow cytometry-based neoantigen-specific T cell sorting; G rapid expansion protocol (REP) of NRT cells, H reinfusion of NRT cells into patients or mouse model