Fig. 8

Gm49317-201 and CDKN1B affects age-related OA in vivo. A Establishment of a rescue model for mice age-related OA targeting Gm49317-201 and CDKN1B. B Representative images of GFP staining revealed the specific adeno-associated virus (AAV) efficiently infected the cartilage of different groups. Scale bar, 200 µm. C Representative images of Safranin O / Fast green staining and OARSI grade use for evaluation of cartilage degeneration in mice cartilage of different groups (n = 10) ** p < 0.01, *** p < 0.001. Scale bar, 200 µm. D 3D reconstruction images of micro-CT scanning of the knees and the number of osteophytes (yellow arrow) (n = 10) ** p < 0.01, *** p < 0.001. E Representative images and Fluorescence intensity of IF staining for p16INK4a in mice cartilage of different groups (n = 10) * p < 0.05. Scale bars, 200 µm and 50 µm