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Fig. 4 | Biomarker Research

Fig. 4

From: LncRNA AC006064.4–201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1

Fig. 4

AC006064.4–201 blocks PTBP1 from binding to CDKN1B mRNA. A RIP assay for PTPB1 levels in HEK-293 T cells transfected with PTBP1-Flag (n = 9, 3 donors for three replicates) ** p < 0.01. B Protein levels of CDKN1B assessed by western blotting analysis when PTBP1 was knocked down or upregulated in HCs. C Protein levels of CDKN1B assessed by western blotting analysis when AC006064.4–201 was knocked down or upregulated in HCs. D MRNA levels of CDKN1B assessed by qRT-PCR when PTBP1 was knocked down or upregulated in HCs (n = 9, 3 donors for three replicates) E MRNA levels of CDKN1B assessed by qRT-PCR when AC006064.4–201 was knocked down or upregulated in HCs (n = 9, 3 donors for three replicates) F Western blotting of CDKN1B when AC006064.4–201 and PTBP1 were simultaneously knocked down or upregulated in HCs. G AC006064.4–201 was upregulated or knocked down in HCs first, and treated with 50 mg/mL CHX for the indicated times, finally the protein levels of CDKN1B were assessed by western blotting analysis. H RIP assay revealed the combination levels between CDKN1B and PTBP1 when AC006064.4–201 was upregulated or knocked down in HEK-293 T cells (n = 9, 3 donors for three replicates) ** p < 0.01, *** p < 0.001

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Biomarker Research

ISSN: 2050-7771

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