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Fig. 3 | Biomarker Research

Fig. 3

From: LncRNA AC006064.4–201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1

Fig. 3

AC006064.4–201 directly interacts with Polypyrimidine tract-binding protein 1 (PTBP1) in HCs. A Five proteins with highest pep score of AC006064.4–201 RPD-MS and their effects on Mmp13, Aggrecan, p21 and p53 assessed by western blotting analysis. B Coomassie brilliant blue staining of proteins binding to AC006064.4–201. C Western blotting of PTPB1 in HCs after pulled-down with the biotinylated AC006064.4–201 mixed probes. D RNA immunoprecipitation (RIP) assay for AC006064.4–201 levels in HEK-293 T cells transfected with PTBP1-Flag (n = 9, 3 donors for three replicates) *** p < 0.001. E RNA–protein colocalization assay confirmed the interaction between AC006064.4–201 and PTBP1 in HCs. Scale bar, 25 µm. F Interacting regions between AC006064.4–201 and PTBP1 predicted by catRAPID tool. G Schematic diagram of truncated AC006064.4–201, and RIP assay identified the binding sequence of AC006064.4–201 for PTBP1 (n = 9, 3 donors for three replicates) *** p < 0.001. H MRNA levels of PTBP1 assessed by qRT-PCR when AC006064.4–201 was knocked down or upregulated in HCs (n = 9, 3 donors for three replicates) I Protein levels of PTBP1 assessed by western blotting analysis when AC006064.4–201 was knocked down or upregulated in HCs

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Biomarker Research

ISSN: 2050-7771

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