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Fig. 2 | Biomarker Research

Fig. 2

From: LncRNA AC006064.4–201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1

Fig. 2

AC006064.4–201 alleviates the senescence of HCs. A MRNA levels of Mmp3, Mmp13, Sox9, Aggrecan, p16INK4a, p21 and p53 assessed by qRT-PCR in HCs after treating with AC006064.4–201 Antisense Oligonucleotides (ASOs) (n = 9, 3 donors for three replicates) * p < 0.05, ** p < 0.01, *** p < 0.001. B Protein levels of Mmp3, Mmp13, Sox9, Aggrecan, p21 and p53 assessed by western blotting in HCs after treating with AC006064.4–201 ASOs. C Representative images of β-galactosidase staining in HCs after treating with AC006064.4–201 ASOs. D Representative photomicrographs of IF staining of p16INK4a and Col2a1 when AC006064.4–201 was downregulated in HCs. Scale bar, 50 µm. E Quantification of SA-β-Gal staining and fluorescence intensity of IF staining of p16INK4a and Col2a1 in the above three groups. * p < 0.05, ** p < 0.01. F MRNA levels of Mmp3, Mmp13, Sox9, Aggrecan, p16INK4a, p21 and p53 assessed by qRT-PCR when AC006064.4–201 was upregulated in HCs (n = 9, 3 donors for three replicates) * p < 0.05, ** p < 0.01, *** p < 0.001. G Protein levels of Mmp3, Mmp13, Sox9, Aggrecan, p21 and p53 assessed by western blotting when AC006064.4–201 was overexpressed in HCs. H Representative images of β-galactosidase staining in HCs when AC006064.4–201 was overexpressed in HCs. I Representative photomicrographs of IF staining of p16INK4a and Col2a1 when AC006064.4–201 was upregulated in HCs. Scale bar, 50 µm. J Quantification of SA-β-Gal staining and fluorescence intensity of IF staining of p16INK4a and Col2a1 in the above two groups. * p < 0.05, ** p < 0.01

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