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Fig. 1 | Biomarker Research

Fig. 1

From: LncRNA AC006064.4–201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1

Fig. 1

AC006064.4–201 exhibits lower expression in senescent and degenerated HCs. A Heat map of differentially expressed lncRNAs between normal human chondrocytes (HCP0) and senescent human chondrocytes (HCP3). B Quantitative real time (qRT-PCR) of AC006064.4–201 in specific sections of human knee cartilages of different ages (n = 15). * p < 0.05, ** p < 0.01. C Representative images of Safranin O / Fast green staining, FISH staining for AC006064.4–201, and IF staining for p16INK4a and Mmp3 in specific sections of human knee cartilages of different ages. Scale bars, 1 mm, 500 µm and 200 µm. D Expression of AC006064.4–201 in HCs after cheating with different concentrations of Bleomycin (0 ug/ml, 50ug/ml and 100ug/ml) (n = 9, 3 donors for three replicates) *** p < 0.001. E Expression of AC006064.4–201 assessed by qRT-PCR in the nuclear and cytoplasmic fractions. F RNA FISH showed that AC006064.4–201 was predominantly localized in the cytoplasm. Scale bar, 25 µm

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Biomarker Research

ISSN: 2050-7771

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