Fig. 1
From: HMGA1 augments palbociclib efficacy via PI3K/mTOR signaling in intrahepatic cholangiocarcinoma
HMGA1 is a potential activator of iCCA progression
A, Amplification of HMGA1 is common in TCGA and GEO provisional iCCA cohort
B-C, The expression of HMGA1 was detected with qPCR in 18 pairs of iCCAs and adjacent tissues and with WB in four randomly-selected pairs of iCCA tissues
D, HMGA1 expressions in intrahepatic bile duct cell line HiBEpiC and different hepatobiliary tumor cell lines: HUCCT1, HCCC-9810 and RBE iCCA cell lines and PLC/PRF/5, Huh7, HepG2 and Hep3B.hepatocellular carcinoma cell lines
E, Western blot showed that HMGA1 expression was silenced in HUCCT1 cells or overexpressed in RBE cells
F-I, HMGA1 enhanced the proliferation, migration and invasion of iCCA cell lines. Cell proliferation was evaluated with CCK-8 assay and colony formation assay. Cell migration and invasion were investigated with transwell assays
J, Effects of HMGA1 on EMT were detected by western blot after silencing or overexpressing HMGA1 in iCCA cells
K-L, 3D sphere formation and western blot indicated HMGA1 increased the stemness of iCCA.
M-Q, Xenografts and metastasis models were established with stable HMGA1-silenced HUCCT1 cells. Mice engrafted with HMGA1-silencing cells exhibited lower tumor volumes, tumor weights and metastatic lesions
Data were shown as mean ± SEM. n.s. represents not significant. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 as calculated by the one-way or two-way ANOVA. Scale bar: 50 μm