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Fig. 6 | Biomarker Research

Fig. 6

From: RNA demethylase ALKBH5 promotes tumorigenesis of t (8;21) acute myeloid leukemia via ITPA m6A modification

Fig. 6

TCF15 regulates ALKBH5 expression by elevating its promoter activity. A Flowchart of screening for transcription factors. mRNA level of TCF15 (B) or ALKBH5 (C) in CD34+ LSCs/LICs and CD34− bulk AML cells, respectively. D-E CD34+ LSCs/LICs cells were transduced with reporter plasmids containing either wild or mutant ALKBH5 promoter fragments. Then, luciferase activity was measured in CD34+ LSCs/LICs cells transduced with the empty vector or TCF15. qPCR (F) and western blots (G) of TCF15, ALKBH5, and ITPA in LSCs transduced with shNC, shTCF15#1, or shTCF15#2. H Kaplan–Meier curve analysis between high-TCF15 and low-TCF15 groups in TCGA-LAML cohort. The median of TCF15 expression was used as a cut-off value. I Colony-forming cell counts of transduced cells. J Primary cells were transduced with shNC or shTCF15, together with an empty (vector) or ALKBH5-encoding lentivirus. After drug selection, those co-transduced cells were prepared for cell proliferation assays. K The role of TCF15/ALKBH5/ITPA in AML progression, drawn by Figdraw

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