Fig. 5
ALKBH5 regulates ITPA expression by affecting its mRNA stability. A The mRNA half-life (t1/2) of ITPA in Kasumi-1 cells upon ALKBH5 knockdown. B qPCR analysis of the distribution of ITPA, GAPDH mRNA, and MALAT1 RNA in subcellular fractions from Kasumi-1 cells. GAPDH mRNA is predominantly located in the cytoplasm, whereas MALAT1 RNA is predominantly located in the nucleus. C The promoter activity of ITPA in Kasumi-1 cells transduced with shNC or shALKBH5. D ALKBH5-RIP qPCR validation of ALKBH5 binding of ITPA. E Gene-specific m6A-RIP qPCR validation of m6A level changes of ITPA. F The mRNA level of ITPA in Kasumi-1 cells transduced with shNC or shALKBH5, together with an empty (vector) or wild-type ALKBH5-encoding lentivirus or the H204A mutant