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Fig. 2 | Biomarker Research

Fig. 2

From: RNA demethylase ALKBH5 promotes tumorigenesis of t (8;21) acute myeloid leukemia via ITPA m6A modification

Fig. 2

Knockdown of ALKBH5 affects the maintenance of murine and human AML. A Bone marrow lineage-negative (Lin−) cells collected from t (8;21) murine leukemia model were transduced with shNC or shAlkbh5 and used for colony-forming/replating assays (CFAs). Transduced cells were also transplanted into lethally irradiated recipient mice after the first round of colony formation for leukemogenesis. B Colony-forming cell counts of transduced cells. C Kaplan-Meier survival curves of recipient mice transplanted with transduced cells. Percentage of GFP+ cells in peripheral blood (D) and BM (E) of recipient mice. Representative IHC images of the spleen (F) and liver (G) in recipient mice transplanted with leukemic cells with shNC or shAlkbh5. H Kaplan-Meier survival curves of NSG mice transplanted with Kasumi-1 cells that were transduced with shNC (n = 5) or shALKBH5 (n = 5). I Percentage of human CD45+ cells in BM (CDX model). (J) Kaplan-Meier survival curves of NSG mice transplanted with primary t (8;21) AML cells that were transduced with shNC (n = 5) or shALKBH5 (n = 5). (K) Percentage of human CD45+ cells in BM (PDX model)

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