Fig. 7

circEMB affects the malignant behaviour of OSA via the circEMB/miR-3184-5p/EGFR axis. a-c CCK-8 and colony formation assays revealed the changes in proliferation abilities of 143B and U2OS after simultaneous cell transfection with sh-circ-EMB, miR-3184-5p inhibitors, and sh-EGFR. d Transwell assay was performed to examine the effects of the circEMB/miR-3184-5p/EGFR axis on the migration and invasion abilities of cells. Scale bar, 100 μm. e-f Effects of the circEMB/miR-3184-5p/EGFR axis on cell cycle arrest and apoptosis were evaluated by flow cytometry. h Western blotting was used to assess the effects of the circEMB/miR-3184-5p/EGFR axis on the expression of E-cadherin, N-cadherin, Vimentin, PCNA, CDK4, CCND1, BAX, BCL2, Jagged1, and EGFR. i An in vivo imaging system was used to examine the effects of the circEMB/miR-3184-5p/EGFR axis on cell proliferation. j IHC staining for Ki-67, PCNA, Vimentin, N-cadherin and E-cadherin in each group. Scale bar, 100 μm. k An in vivo imaging system was used to examine the effects of the circEMB/miR-3184-5p/EGFR axis on metastasis in nude mice. Scale bar, 1000 μm. l Microscopic images of HE staining of metastatic nodules in the lungs of nude mice. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)