Fig. 6

The circEMB/miR-3184-5p/EGFR axis was activated in OSA cells. a 143B cells transfected with sh-circEMB and scramble shRNA were subjected to RNA-seq. b Schematic representation of the overlap among target mRNAs of miR-3184-5p predicted using the TargetScan, miRanda, RNAhybrid, and miRDB databases. c Schematic representation of the overlap among target mRNAs of miR-3184-5p and mRNAs downregulated via circEMB knockdown. d Pathway enrichment analysis and GSEA revealed that the overlap genes were markedly enriched in the Notch signalling pathway. e-f After transfection of sh-circEMB into 143B and U2OS cells, the relative expression of four candidate mRNAs was determined via qRT-PCR. g-h Verification of the binding between miR-3184-5p and EGFR in HEK293T cells by dual-luciferase reporter gene assay. i-j Correlation between the mRNA expression of circEMB and EGFR and between that of miR-3184-5p and EGFR in 53 OSA samples. k Expression of EGFR in paired OSA and adjacent normal tissues was examined via western blotting. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)