Fig. 5

miR-124-3p inhibits NSCLS progression partially through PI3K/AKT signaling pathway. a Volcano plot presenting differentially expressed genes with miR-124-3p mimic transfection. Blue dots represent the downregulated genes (fold change < 0.5, FDR ≤ 0.05) and red dots represent the upregulated genes (fold change > 2, FDR ≤ 0.05). b The heatmap of differentially abundant genes obtained from the mimic NC and miR-124-3p mimic groups. c Significantly downregulated GO pathways with miR-124-3p mimic transfection. d The tumor invasion and migration pathway KEGG pathways enrichment in miR-124-3p mimic groups. e Significantly downregulated KEGG pathways with miR-124-3p mimic transfection. f Western blot to detect protein expression of AKT, p-AKT, PI3K and p-PI3K in A549 and NCI-H1299 cells transfected with mimic NC, miR-124-3p mimic,740 Y-P (20 μM) or miR-124-3p mimic+ 740 Y-P (20 μM). Full-length blots are presented in Additional file 3. g Transwell assays performed in A549 cells transfected with miR-124-3p mimic, 740 Y-P (20 μM) or miR-124-3p mimic + 740 Y-P (20 μM). h Transwell assays performed in NCI-H1299 cells transfected with miR-124-3p mimic, 740 Y-P (20 μM) or miR-124-3p mimic + 740 Y-P (20 μM)