Fig. 5

SLC25A21-AS1 directly regulates PTBP3. a The effect of knockdown or overexpression of SLC25A21-AS1 on the expression of PTBP3 was detected by Western blot (WB). b-c Observe the protein degradation within 12 hours after adding CHX. d-e After knockdown or overexpression of SLC25A21-AS1 and adding proteasome inhibitor (Mg132), the stability of PTBP3 was increased compared with the control group. f-g Ubiquitination assay to detect the ubiquitination level of PTBP3 in SLC25A21-AS1 knockdown or overexpressing EOC cells. Borz (250nM) and NEM (5μm) were added for 6h. h Using catRAPID to predict the binding sites of PTBP3 and SLC25A21-AS1. i The enrichment between PTBP3 and different truncations was detected by RIP assay. The enrichment of T2 was significantly better than that of other truncations (n=3). **P<0.01, *P<0.05. j Electrophoretic mobility shift analysis (EMSA) to verify the interaction between T2 truncation and its mutants with PTBP3. k Ubiquitination assay between different truncations and PTBP3. Borz (250nM) and NEM (5μm) were added for 6h