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Fig. 3 | Biomarker Research

Fig. 3

From: Long non-coding RNA SLC25A21-AS1 inhibits the development of epithelial ovarian cancer by specifically inducing PTBP3 degradation

Fig. 3

The RNA-binding protein PTBP3 was screened and identified. a RNA pull-down experiments and silver staining to find specific bands. Specific band is marked in orange circles. b RNA-pulldown assay to identify candidate proteins from MS analysis. c RIP experiments confirmed that PTBP3 was better enriched when SLC25A21-AS1 was overexpressed (n=3). *P<0.05. d IF-FISH assay confirmed the co-localization between SLC25A21-AS1 and PTBP3, and PTBP3 was mainly present in the cytoplasm, but also partly in the nucleus. Blue represents the nucleus, red represents SLC25A21-AS1, and green represents PTBP3. e The expression of PTBP3 in EOC cells was higher than that in normal ovarian cells

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