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Fig. 1 | Biomarker Research

Fig. 1

From: Long non-coding RNA SLC25A21-AS1 inhibits the development of epithelial ovarian cancer by specifically inducing PTBP3 degradation

Fig. 1

Discovery and identification of SLC25A21-AS1. a Screening according to GSE135886 and GSE14407. b Intersection of down-regulated genes in the two datasets. c SLC25A21-AS1 showed low expression in tumors in both datasets. The clinical data analysis was identified by package Limma using R software (version3.6.2). d Validation in tissue showed that the expression of SLC25A21-AS1 in EOC tissues and even metastatic tissues was significantly lower than that in normal ovarian tissue. e Validation of SLC25A21-AS1 expression levels in different cell lines. According to the difference in expression, two cell lines with moderate expression, SKOV3 and 3AO, were selected. f FISH assay were performed to verify the localization of SLC25A21-AS1 in EOC cells. SLC25A21-AS1 is labeled in both the nucleus and cytoplasm, but more in the cytoplasm. Blue represents nuclei and red represents SLC25A21-AS1. g Nucleocytoplasmic separation experiments confirmed the localization relationship of SLC25A21-AS1 in EOC cells. Error bars are the mean ± SEM of three independent replicate experiments. ***P<0.001

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Biomarker Research

ISSN: 2050-7771

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