Skip to main content
Fig. 3 | Biomarker Research

Fig. 3

From: Loss of miR-637 promotes cancer cell stemness via WASH/IL-8 pathway and serves as a novel prognostic marker in esophageal squamous cell carcinoma

Fig. 3

miR-637 inhibits IL-8 expression and cancer stemness in a WASH-dependent manner. KYSE70 cells stably transduced with negative control shRNA (shNC) or WASH shRNA (shWASH) were treated with NC inhibitor or miR-637 inhibitor. A, B qRT-PCR (A) or Western blotting (B) assays showed the failure of miR-637 inhibitor to induce WASH expression in KWSE70 cells with WASH knockdown. C, D qRT-PCR (C) or tumor sphere formation (D) assays showed the failure of miR-637 inhibitor to induce IL-8 expression and sphere formation capacity in KWSE70 cells with WASH knockdown, respectively. Scale bar, 200 μm. E The relative expression of stemness markers were measured by qRT-PCR. Data are presented as mean ± standard deviation. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page

Biomarker Research

ISSN: 2050-7771

Contact us