Fig. 2
From: CAR-T cell potency: from structural elements to vector backbone components
A schematic picture of TanCAR (a), DualCAR (b), and Loop CAR (c). In TanCAR, two different scFvs are connected outside the cell (in series), usually by a glycine-serine linker. TanCAR can be activated when any one of the scFvs binds to a target antigen. Dual CAR-T cells refer to the expression of two CARs in the same T cell, with each CAR having its own signaling function and distinct extracellular antigen recognition domains. Like TanCAR-T cells, loop CAR-T cells consist of two scFvs in a single CAR molecule. In TanCARs, the VL-VH of one scFv is directly linked to the VL-VH of the other scFv, whereas the loop structure is formed with the VL-VH of one scFv separated by the VL-VH of the other scFv