Fig. 4

HOXC-AS3 Interacts with CDK2. A Relative level of HOXC-AS3 in the nuclear and cytoplasmic fractions of HLF and 97H cells. B HOXC-AS3 interacting with CDK2 peptides in the mass spectrum results after RNA pulldown assay. C Knockdown of HOXC-AS3 did not significantly inhibit CDK2 expression on transcription level in HLF and Hep3B cells. D Overexpressing of HOXC-AS3 did not significantly inhibit CDK2 expression on transcription level in HLF and 97H cells. E Protein level of CDK2 in HLF, Hep3B and 97H cells after transfected with Aso-HOXC-AS3 or pcDNA-HOXC-AS3 plasmids. F FISH assay to verify the expression localization of HOXC-AS3 and CDK2. G RNA pull-down assay to confirm the association between HOXC-AS3 and CDK2. H RIP assay to confirm the association between HOXC-AS3 and CDK2 in HLF and 97H cells. Data represent the MEAN ± S.E.M of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t test)