Fig. 5

LIPH-4 regulates IGF2BP2 through miR-216b in ESCC cells. A Sequence alignment of miR-216b with binding sites in the wild-type (IGF2BP2-wt) and mutant-type regions of IGF2BP2 (IGF2BP2-mut). B Relative expression levels of IGF2BP2 in ESCC and adjacent noncancerous tissue samples assessed by qRT-PCR (n = 53). C Negative correlation between miR-216b and IGF2BP2. D Positive correlation between LIPH-4 and IGF2BP2. E Relative luciferase activity in 293 T cells was assessed after co-transfection with the reporter plasmid (IGF2BP2-wt or IGF2BP2-mut) and miRNAs (miR-216b or NC mimics) (n = 3). F IGF2BP2, cyclinD1, AKT, p-AKT protein levels in KYSE150 cells transfected with LIPH-4 and in KYSE510 cells transfected with si-LIPH-4, assessed by immunoblot. G IGF2BP2 protein levels in KYSE150 cells transfected with LIPH-4 and miR-216b mimic, and KYSE510 cells transfected with si-LIPH-4 and miR-216b inhibitor, measured by immunoblot. *P < 0.05, **P < 0.01, ***P < 0.001