Fig. 7

Overexpression of DNAJB4 inhibits the proliferation but promotes apoptosis abilities of NSCLC cells. A DNAJB4 mRNA expression in HCC827 and A549 cells transfected with pcDNA4.0 vector, pcDNA4.0- DNAJB4 vector, CTRL‑shRNA, or DNAJB4 ‑shRNA were determined by RT-qPCR. B DNAJB4 protein expression in HCC827 and A549 cells transfected with indicated vectors, which divided into five groups, including Control, OE-CTRL, OE-DNAJB4, shCTRL and shDNAJB4. C CCK8 assay was used to compare the cell proliferation of CTRL, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells. D Edu assay of Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells, respectively. E FSC assay to detect cell apoptosis of Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells. F Expression level of Bax, Bcl-2 and Cytochrome C in the HCC827 and A549 cells of the 5 groups (Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups) were determined by western blotting. Control negative control, OE over expression. Data represent mean values ± SD from three replicates of each sample; **P < 0.01, ***P < 0.001, ****P < 0.001