Fig. 6

DNAJB4 is a direct target gene of miR-148a-3p. A DNAJB4 acted as a potential target of miR-148a-3p using bioinformatics analysis. B RT-qPCR was used to quantify DNAJB4 expression in NSCLC tissues as well as normal lung tissues, n = 132. C Representative images of circ_0009043 IHC staining of NSCLC and normal tissues (× 200, scale bar 100 μm), respectively. D The DNAJB4 protein expression level was significantly suppressed in NSCLC tissues compared with adjacent normal tissues in selected 24 paired tissues. E The expression level of DNAJB4 mRNA using RT-PCR in NSCLC and normal tissues (n = 24); F Correlations between DNAJB4 and circ_0009043. G Correlations between DNAJB4 and miR-148a-3p. H RT-qPCR analysis showed that circ_0009043 overexpression increased DNAJB4 level. I The mRNA level of DNAJB4 in A549 and HCC827 ells transfected with miR-148a-3p mimics or miR-148a-3p inhibitor was measured via RT-qPCR assay. J Online bioinformatics analysis showed the binding within that of miR-148a-3p in addition to 3’-UTR of DNAJB4. K The activity of DNAJB4-wt plasmid in NSCLC cells was suppressed by the mimic transfection of miR-148a-3p which was demonstrated by luciferase reporter assay. *P < 0.05, **P < 0.01, ***P < 0.001