Fig. 3

Circ_0009043 functioned as a sponge for miR-148a-3p in NSCLC cells. A RT-qPCR was performed for ascertaining the expression of miR-148a-3p in NSCLC tissues compared with normal lung tissues. (B) Correlations between circ_0009043 and miR-148a-3p. C Online bioinformatics analysis showed the binding within that of miR-148a-3p in addition to 3’-UTR of circ_0009043. D Expression level of miR-148a-3p in the HCC827 and A549 cells of the 5 groups (CTRL, OE-CTRL, OE- Circ, shCTRL and shCirc groups) were determined by RT-qPCR. E–F Determination of the physical interaction between circ_0009043 and miR-148a-3p by RNA immunoprecipitation experiments. G The activity of circ_0009043-wt plasmid in A549 cells was suppressed by the mimic transfection of miR-148a-3p which was demonstrated by luciferase reporter assay. ***P < 0.001; ****P < 0.0001