Skip to main content
Fig. 2 | Biomarker Research

Fig. 2

From: CircRNA 0009043 suppresses non-small-cell lung cancer development via targeting the miR-148a-3p/DNAJB4 axis

Fig. 2

Overexpression of circ_0009043 inhibits the proliferation, while accelerates apoptosis of NSCLC cells. A circ_0009043 mRNA expression in HCC827 and A549 cells transfected with pcDNA4.0 vector (OE-CTRL), pcDNA4.0- circ_0009043 vector (OE-Circ), CTRL‑shRNA (shCTRL), or circ_0009043‑shRNA (shCirc) were determined by RT-qPCR. B CCK8 assay was used to compare the cell proliferation of Control, OE-CTRL, OE-Circ, shCTRL, and shCirc groups in HCC827 and A549 cells. C Edu assay of Control, OE-CTRL, OE-Circ, shCTRL, and shCirc groups in HCC827 and A549 cells. D-E The ratio of apoptosis in the HCC827 and A549 cells transfected with indicated vectors, which consisting of the OE-CTRL, OE- Circ, shCTRL and shCirc groups were detected by flow cytometry. Comparison of the ratio of apoptosis in the afore mentioned 5 groups. Each bar indicates the mean apoptosis rate ± standard deviation per group. F-G Expression level of Bax, Bcl-2 and Cytochrome C in the HCC827 and A549 cells of the 5 groups (CTRL, OE-CTRL, OE- Circ, shCTRL and shCirc groups) were determined by western blotting. Data was normalized to GAPDH. All results were representative of three separate experiments. Data represent mean values ± SD from three replicates of each sample; *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page