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Fig. 5 | Biomarker Research

Fig. 5

From: 5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

Fig. 5

HNRNPK suppresses TRAF3IP2 via DNA methylation via recruiting DNMT1. A-B 786-O cells transfected with Flag-HNRNPK/Flag-DNMT1 and UOK109 cells were lysed, then CoIP assay was performed with anti-HNRNPK antibody, anti-Flag antibody, anti-DNMT1 antibody or normal rabbit IgG. C-D HEK 293 T cells were transfected with DNMT1 truncations or HNRNPK truncations, then CoIP assay was performed. E The CpG island located in the promoter region of TRAF3IP2 was predicted by MethPrimer. F Bisulfite PCR of CpG island located in the promoter region of TRAF3IP2 following bisulfite conversion in UOK109 cells and 786-O cells transfected with indicated lentivirus. G-J UOK109 cells and 786-O cells were transfected with indicated lentivirus, then MeDIP assay was performed with anti-5mC antibody. K-N UOK109 cells and 786-O cells were transfected with indicated lentivirus or treatment with indicated inhibitors, then the mRNA level of TRAF3IP2 was analyzed. O-Q The mRNA and protein levels of TRAF3IP2 and DNMT1 were detected in UOK109 cells and 786-O cells transfected shDNMT1. R Schematic illustration of dCas9-based system to deliver the exogenous expressed protein to the promoter of TRAF3IP2. S-U The mRNA and protein levels of TRAF3IP2 were detected in UOK109 cells and 786-O cells transfected dCas9-DNMT1 and gTRAF3IP2. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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