Fig. 7

ALDH1A3 promotes DTX resistance via activating PI3K/AKT/mTOR signaling pathway. A The IC50 values of DU145-DR cells were detected after treatment with 20 nM DTX and transfection with si-ALDH1A3. B-E Flow cytometry analysis were used to assess cell apoptosis (B, C) and cell cycle (D, E) in DU145-DR cells treated with 20 nM DTX after transfection of si-ALDH1A3. F-I The protein levels of PI3K and downstream molecules were determined in those cells co-transfected with siRNAs or overexpression plasmid and miR-1301-3p inhibitors by western blot. All data are presented as the means ± SD of three independent experiments. n = 3, **P < 0.01, ***P < 0.001