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Fig. 6 | Biomarker Research

Fig. 6

From: circCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3

Fig. 6

CircCYP24A1 upregulates ALDH1A3 expression by post-transcriptional mechanisms in DTX-resistant PCa. A Venn diagram visualizing the overlap of 13 genes which was predicted according to the cross-analysis of PITA, miRanda, ENCORI, TargetScan and the mRNA-seq results. B, D The expression of those candidate genes after transfected with miR-1301-3p mimic (B) or inhibitor (D). C The enrichment of ALDH1A3 mRNA was detected by biotinylated miRNA pull-down in DU145-DR and DU145 cells. E Anti-AGO2 RIP assays were performed in DU145 cells, and the enrichment of ALDH1A3 was assessed by qRT-PCR. F The luciferase activities were determined in HEK293T cells co-transfected with ALDH1A3 luciferase reporter vectors and miR-1301-3p mimics or mimic NC (bottom), and the schematic showed wild-type (WT) and mutant (MUT) ALDH1A3 luciferase reporter vectors (upper). G-L The RNA and protein levels of ALDH1A3 was detected by qRT-PCR or western blot in indicated cells, respectively. M IHC score of ALDH1A3 in DTX-sensitive (n = 30) and DTX-resistant (n = 40) PCa biopsy tissues. N Representative IHC images of ALDH1A3 in three DTX-resistant/−sensitive patients, respectively. All data are presented as the means ± SD of three independent experiments. n = 3, **P < 0.01. ***P < 0.001

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