Fig. 2

CircCYP24A1 enhances DTX resistance in vitro. A Schematic illustration of two sh-circCYP24A1 RNAs targeted the back-splicing junction. B-D qRT-PCR analysis of circCYP24A1 and CYP24A1 mRNA expression in DU145-DR (B), DU145 (C) and 22Rv1 (D) cells transfected with indicated lentivirus. E-G CCK-8 assays were used to detected the DTX cytotoxicity after down−/up-regulation of circCYP24A1 in DU145-DR (E), DU145 (F) or 22Rv1 (G) cells. H-J The indicated cells were treated with DTX (20 nM for DU145-DR/DU145 and 3 nM for 22Rv1) or PBS (control) and subjected to Annexin V-APC and PI stanning to detect apoptotic rate by flow cytometry. K-M Cell cycle were detected by flow cytometry in indicated cells after treatment with DTX (20 nM for DU145-DR/DU145 and 3 nM for 22Rv1). All data are presented as the means ± SD of three independent experiments. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001