Skip to main content
Fig. 1 | Biomarker Research

Fig. 1

From: circCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3

Fig. 1

CircCYP24A1 was identified and characterized in DTX-resistant PCa. A Volcano plots visualizing 107 upregulated and 67 downregulated circRNAs in DU145-DR cells. B The chromosomal localization of those significantly expressed circRNAs. C Hot map visualizing 7 upregulated and 4 downregulated circRNAs in DU145-DR cells according to the log2 (fold-change) > 1 or < − 1 and q value < 0.05. D Basic information for the dysregulated circRNAs. E qRT-PCR analysis of the dysregulated circRNAs in DU145-DR and DU145 cells. F PCR and agarose gel electrophoresis analysis were used to verify the existence of linear and circular forms of CYP24A1 in cDNA and gDNA samples from DU145-DR cells. G Schematic illustration of circCYP24A1 back splicing junction validated by Sanger sequencing. H ActD treatment was used to evaluate the stability of circCYP24A1 and CYP24A1 mRNA in DU145-DR cells. I qRT-PCR analysis of the circCYP24A1 and CYP24A1 mRNA level after RNase R exonuclease treatment. J Nuclear-cytoplasmic fractionation assay was performed to observe the subcellular distribution of circCYP24A1 in DU145-DR cells. The ACTB mRNA and U6 snRNA were used as cytoplasmic and nuclear markers, respectively. K The circCYP24A1 probe labeled with Cy3 (red) was used to visualize the location of circCYP24A1 in DU145-DR cells via FISH assay, and the nuclei were stained with DAPI (blue). L FISH score of circCYP24A1 in DTX-sensitive (n = 30) and DTX-resistant (n = 40) PCa biopsy tissues. M Representative FISH images of circCYP24A1 in three DTX-resistant/−sensitive patients, respectively. The circCYP24A1 probe was labeled with Cy3 (red), and the nuclei were stained with DAPI (blue). All data are presented as the means ± SD of three independent experiments. n = 3, ***P < 0.001

Back to article page