Fig. 3

Linear modeling and differential expression of cLEC extracellular miRNAs in response to varying ΔP63α^bright cell percentages. a cLEC ΔP63α^bright cell percentage distribution across 72 cLECs. Red dots represent the 18 samples selected for HTG EdgeSeq miRNA whole transcriptome assay. b Plot distribution of quantile transformed miRNA counts against ΔP63α^bright cell percentages. Linear trend for the top significantly regulated targets miR-6723-5p (left panel), miR-6075 (right panel - green) and miR-4649-5p (right panel - red). c PCA plot of the miRNA data characterizing the trends exhibited by the expression profiles of ΔP63α^bright LOW (blue) and HIGH (orange) cLEC conditioned media samples. Unconditioned CTRL media are depicted in black. d Top 4 most differentially expressed miRNAs in cLEC conditioned media from LOW and HIGH ΔP63α^bright cell groups