Fig. 2
cLEC characterization and phenotyping. a Representative picture of the epithelial morphology observed in cLECs as seen by contrast phase microscopy (10X). b Average cell density obtained from the 624 graded areas of 40, 000μm2 (8 areas/cLEC). c Software detection and quantification of small cells with diameter inferior to 12 μm (10X). d Phenotypic characterization and quantification of cLSCs using immunofluorescent labelling of K12 (red), K14 (green) and Hoechst dye (blue); e Vimentin (red), PanCytokeratin (green) and Hoechst dye (blue); f ΔP63α (red) and Hoechst dye (blue). Representative pictures as seen by fluorescent microscopy (20X). Scale bar is set at 50 μm